Practical/Laboratory Manual Biology Class XII based on NCERT guidelines by Dr. Sunita Bhagia & Megha Bansal

Book excerpt: A. List of Experiments 1.Study pollen germination on a slide, 2.Collect and study soil from at least two different sites and study them for texture, moisture content, pH and water holding capacity. Correlate with the kinds of plants found in them, 3. Collect water from two different water bodies around you and study them for pH, clarity and presence of any living organism, 4. Study the presence of suspended particulate matter in air at two widely different sites, 5. Study the plant population density by quadrate method, 6. Study the plant population frequency by quadrate method, 7. Prepare a temporary mount of onion root tip to study mitosis. 8. Study the effect of different temperatures and three different pH on the activity of salivary amylase on starch. 9. Isolate DNA from available plant material such as spinach, green pea seeds, papaya, etc. B. Study/observation of the following (Spotting) 1. Flowers adapted to pollination by different agencies (wind, insects, birds). 2. Pollen germination on stigma through a permanent slide. 3. Identification of stages of gamete development, i.e., T.S. of testis and T.S. of ovary through permanent slides (from grasshopper/mice). 4. Meiosis in onion bud cell or grasshopper testis through permanent slides. 5. T.S. of blastula through permanent slides (Mammalian). 6.Mendelian inheritance using seeds of different colour/sizes of any plant.7. Prepare pedigree charts of any one of the genetic traits such as rolling of tongue, blood groups, ear lobes, widow's peak and colour blindness. 8. Controlled pollination-emasculation, tagging and bagging. 9. Common disease causing organisms like Ascaris, Entamoeba, Plasmodium, any fungus causing ringworm through permanent slides or specimens. Comment on symptoms of diseases that they cause. 10. Two plants and two animals (model/virtual images) found in xeric conditions. Comment upon their morphological adaptations. 11. Two plants and two animals (models/virtual images) found in aquatic conditions. Comment Content EXPERIMENTS 1.To study pollen germination on slide. 2. To study the texture moisture content pH and waterHolding Capacity of soils collected from different sites. 3.To collect water from different water bodies and study them for pH Clarity and presence of living organisms. 4. To study the presence of suspended particulate matter in air at different sites. 5.To study plant population density by quadrat method.6.To study plant population frequency by quadrat method. 7.To study various stages of mitosis in root tip of onion by preparing slide in acetocarmine. 8.To study effect of different temperature and three different pH onthe activity of salivary amylase. 9. To study the isolation of DNA from available plant material such as spinach green pea,seeds, papaya etc. SPOTTING 1.Pollination in flowers. 2. Pollen germination. 3.Slides of mammal tissues. 4. Meiosis cell division. 5. T. S. of Blastula. 6. Mendel's inheritance laws. 7. Pedigree chart. 8. Controlled pollination. 9.Common disease causing organisms. 10. Xerophytic adaptation. 11.Aquatic adaptation.