Pichia Protocols
Download or Read eBook Pichia Protocols PDF written by James M Cregg and published by Springer Science & Business Media. This book was released on 2007-08-08 with total page 553 pages. Available in PDF, EPUB and Kindle.
| Author | : James M Cregg |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 553 |
| Release | : 2007-08-08 |
| ISBN-10 | : 9781588294296 |
| ISBN-13 | : 1588294293 |
| Rating | : 4/5 (96 Downloads) |
Book Synopsis Pichia Protocols by : James M Cregg
Book excerpt: This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
