OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay
Download or Read eBook OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay PDF written by OECD and published by OECD Publishing. This book was released on 2021-06-18 with total page 51 pages. Available in PDF, EPUB and Kindle.
| Author | : OECD |
| Publisher | : OECD Publishing |
| Total Pages | : 51 |
| Release | : 2021-06-18 |
| ISBN-10 | : 9789264714137 |
| ISBN-13 | : 9264714138 |
| Rating | : 4/5 (37 Downloads) |
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay by : OECD
Book excerpt: The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.
