Molecular Methods for the Identification and Uantification of Cyanobacteria in Surface Water Sources
Author | : Treyton Michael Moore |
Publisher | : |
Total Pages | : 55 |
Release | : 2019 |
ISBN-10 | : OCLC:1102451999 |
ISBN-13 | : |
Rating | : 4/5 (99 Downloads) |
Book excerpt: This study investigates the use of quantitative Polymerase Chain Reaction (qPCR) for the early detection of geosmin-producing cyanobacteria. qPCR can detect geosmin-producing cyanobacteria via their DNA. I developed a qPCR assay for this study that is capable of sensitively detecting multiple strains of the geosmin-producing Nostoc genus. The developed assay showed high sensitivity, demonstrating the possibility for its use in detecting low concentrations of geosmin-producing cyanobacteria before detectible levels of geosmin have been produced and released into the water. Through further sequencing of more geosmin-producing genera and species, the methodology outlined in this research could be applied to develop the tools necessary to predict taste-and-odor events caused by geosmin-producing cyanobacteria.