CRISPR-Cas Methods
| Author | : M. Tofazzal Islam |
| Publisher | : Humana |
| Total Pages | : 397 |
| Release | : 2021-07-31 |
| ISBN-10 | : 1071616560 |
| ISBN-13 | : 9781071616567 |
| Rating | : 4/5 (60 Downloads) |
Book excerpt: This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism. The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
