Regulation of Ribosome Biogenesis and Rna Polymerase I Transcription
Author | : Robert Steinbauer |
Publisher | : Sudwestdeutscher Verlag Fur Hochschulschriften AG |
Total Pages | : 148 |
Release | : 2011-06 |
ISBN-10 | : 3838126149 |
ISBN-13 | : 9783838126142 |
Rating | : 4/5 (49 Downloads) |
Book excerpt: Ribosome synthesis depends on nutrient availability sensed by the target of rapamycin (TOR) signaling pathway in eukaryotic cells. TOR inactivation affects ribosome biogenesis at the level of RNA polymerase I (Pol I)-dependent transcription of ribosomal RNA (rRNA) genes, expression of ribosomal proteins (r-proteins) and ribosome biogenesis factors, pre-ribosome processing, and transport. Detailed analysis shows that upon TOR inactivation the levels of newly synthesized ribosomal subunits drop drastically before the integrity of the Pol I apparatus is severely impaired but in good correlation with a sharp decrease in r-protein production. Inhibition of translation by cycloheximide mimics the rRNA maturation defect observed immediately after TOR inactivation. Both cycloheximide addition and the depletion of individual r-proteins also reproduce TOR-dependent nucleolar entrapment of specific ribosomal precursor complexes. The conclusion could be drawn that shortage of newly synthesized r-proteins after short-term TOR inactivation is sufficient to explain most of the observed effects on ribosome production.